Description

ThePSCCryopreservationKitcontainsxeno-freePSCCryopreservationMedium,whichisaready-to-usesolutionforthecryopreservationofearlypassagepluripotentstemcells(PSCs),andRevitaCell™Supplement(100X),achemicallydefinedrecoverysupplementforuseinthepost-thawculturemedia.Whenusedincombination,thesereagentshelpminimizelossofcellviABIlity,maximizepost-thawrecovery,andminimizeunwanteddifferentiationofpluripotentstemcells.Thiskitcanalsobeusedtocryopreserveandrecoverperipheralbloodmononuclearcells(PBMCs)toimprovepost-thawcellviabilityandrecovery.•Efficientrecovery—routinelyachieves50%moreviablecellsthantrADItionalmethodsofPSCcryopreservation•Consistent—chemicallydefinedmediamaintainsnormalmorphology,pluripotency,andkaryotypeofpluripotentstemcellsover10passages•Reducedvariability—optimizedreagentsminimizethecausesofgeneticinstabilityofpluripotentstemcellsReliableXeno-free,chemicallydefinedformulationshelpensuretheproliferationcapacityandlong-termcultureofPSCsandPBMCsinastablestate,thushelpingtoincreasetheirusefulnessinresearchandclinicalresearchapplications.EasytouseThePSCCryopreservationMediumisprovidedasaready-to-use,completesolutioncontaining10%DMSOforefficientcelladdition.Separateadditionsofenhancingreagentsarenotrequired,helpingsavevaluablepreparationtime.VersatileThekitiscompatIBLewithcellsculturedinavarietyofmediasystemsincludingEssential8™medium,StemPro®hESCSFM,andothercommerciallyavailableformulations.Also,thekitiscompatiblewithclumpandsinglecellpassagedPSCs.cGMPmanufacturingandqualitysystemThePSCCryopreservationKitismanufacturedatacGMP-compliantfacility.ThefacilityisalsoregisteredwiththeFDAasamedicaldevicemanufacturerandcertifiedtoISO13485standards.