Description
TrypLE™Expressisananimalorigin-free,recombinantenzymeusedfordissociatingawiderangeofadherentmammaliancells,includingCHO,HEK293,A529,primaryhumankeratinocytes,andembryonicstemcells.TrypLE™ExpresscleavespeptidebondsontheC-terminalsidesoflysineandarginine,andisadirectreplacementfortrypsin.Itsexceptionalpurityincreasesspecificityandreducesdamagetocellsthatcanbecausedbyotherenzymespresentinsometrypsinextracts.
WeofferavarietyofTrypLE™formulationsforarangeofcellcultureapplications.
Comparedtotrypsinandotherdissociationreagents,
Gibco®TrypLE™Expressis:
Gentleoncells•
Room-temperaturestable•
Easytouse•
Animalorigin-free(AOF)ThisTrypLE™Expressismodifiedasfollows:
TheformulationofTrypLE™solutionsisavailable,althoughwekeeptheenzymeconcentrationconfidential.
GentleOnCellsTrypLE™Express"exceptionalpurityincreasesspecificityduetotheactionofasingleenzyme.Thisreducesdamagecausedbycleavagefromenzymespresentinsometrypsinpreparationsandinotherproteaseextracts.
Room-TemperatureStableTrypLE™Expressisroom-temperaturestableandreadytouse.TrypLE™Expressremainsstablefor24monthsatroomtemperatureor2–8°C,makingstorageandhandlingeasyandconvenient.
EasytoUseTrypLE™Expresscanbedirectlysubstitutedfortrypsininexistingprotocols.Inaddition,dilutionaloneinactivatesTrypLE™Express,avoidingtheneedfortrypsininhibitors,suchasFBS.
AnimalOrigin-Free(AOF)Unlikeporcinetrypsin,TrypLE™Expressisfreeofanimal-derivedcomponents.
cGMPManufacturingandQualitySystemForsupplychaincontinuity,wemanufactureGibco®TrypLE™ExpressattwoseparatefacilitieslocatedinGrandIsland,NYandScotland,UK.BothsitesarecompliantwithcGMPmanufacturingrequirements,arecertifiedtotheISO13485standard,andareregisteredwiththeFDAasmedicaldevicemanufacturers.
ForResearchUseOnly.Notforuseindiagnosticprocedures.