Description
TrypLE™Selectisananimaloriginfree,recombinantenzymefordissociatingawiderangeofadherentmammaliancells,includingCHO,HEK293,A529,primaryhumankeratinocytes,andembryonicstemcells.WeofferavarietyofTrypLE™formulationsforarangeofcellcultureapplications.
Comparedtotrypsinandotherdissociationreagents,Gibco®TrypLE™Selectis:•
Gentleoncells—maintainscellhealthforreproduc
IBLeresults
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Room-temperaturestable—convenienttouse
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Easytouse—substitutesdirectlyintoexistingprotocols,withnoinactivationrequired
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Animaloriginfree(AOF)—pure,consistent,andeffective
ThisTrypLE™Selectismodifiedasfollows:
TheformulationofTrypLE™solutionsisavailable,althoughwekeeptheenzymeconcentrationconfidential.
GentleoncellsTrypLE™Select,liketrypsin,cleavespeptidebondsontheC-terminalsidesoflysineandarginine.However,TrypLE™Select"sexceptionalpurityincreasesspecificityduetotheactionofasingleenzyme.Thisreducesdamagecausedbycleavagefrommultipleenzymesintrypsinandotherextracts.
Room-temperaturestableTrypLE™Selectisroom-temperaturestableandreadytouse.TrypLE™Selectcelldissociationreagentsremainstablefor24monthsatroomtemperatureor2–8°C,makingstorageandhandlingeasyandconvenient.
EasytouseTrypLE™Selectcanbesubstitutedfortrypsininexistingprotocols.Inaddition,dilutionaloneinactivatesTrypLE™Select,avoidingtheneedfortrypsininhibitors,suchasFBS.
AnimalOriginFree(AOF)Unlikeporcinetrypsinandotherenzymes,TrypLE™Selectisfreeofanimal-derivedcomponents,andisformulatedondedicatedanimalorigin-freeequipment.
ProductIntendedUseForResearchUseorFurtherManufacturing.Notforuseindiagnosticprocedures.CustomersusingGibco®TrypLE™Selectinamanufacturingprocess,whohaveasubmissionwiththeFDA,mayrequestaletterofauthorizationfromustoreferenceourTypeIIDrugMasterFile(DMF).
cGMPManufacturingandQualitySystemGibco®TrypLE™SelectismanufacturedatacGMPcompliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
ConcentratedformsofTrypLE™canbeasepticallydilutedto1xusingabalancedsaltsolutioncontaining1mMEDTA.
ForResearchUseorFurtherManufacturing.Notforuseindiagnosticprocedures.