Description

RPMI1640Medium(RoswellParkMemorialInstitute1640Medium)wasoriginallydevelopedtoculturehumanleukemiccellsinsUSPensionandasamonolayer.RPMI1640mediumhassincebeenfoundsuitableforavarietyofmammaliancells,includingHeLa,Jurkat,MCF-7,PC12,PBMC,astrocytes,andcarcinomas.WeofferavarietyofRPMI1640modificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
Thecompleteformulationisavailable.

UsingRPMI1640Medium
RPMI1640mediumisuniquefromothermediabecauseitcontainsthereducingagentglutathioneandhighconcentrationsofvitamins.RPMI1640mediumcontainsbiotin,vitaminB₁₂,andPABA,whicharenotfoundinEagle"sMinimalEssentialMediumorDulbecco"sModifiedEagleMedium.Inaddition,thevitaminsinositolandcholinearepresentinveryhighconcentrations.RPMI1640mediumwithGlutaMAX™supplementminimizestoxicammoniabuild-upandimprovescellviABIlityandgrowthinaneasy-to-useformat.RPMI1640mediumcontainsnoproteins,lipids,orgrowthfactors.Therefore,RPMI1640mediumrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).RPMI1640mediumusesasodiumbicarbonatebuffersystem(2.0g/L)andthereforerequiresa5–10%CO₂environmenttomaintainphysiologicalpH.

Productintendeduse
Forinvitrodiagnosticuse.CAUTION:notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.CustomersusingGibco®RPMI1640inamanufacturingprocess,whohaveasubmissionwiththeFDA,mayrequestaletterofauthorizationfromustoreferenceourTypeIIDrugMasterFile(DMF).

cGMPmanufacturingandqualitysystem
RPMI1640ismanufacturedatacGMP-compliantfacilitylocatedinPaisley,Scotland,UK.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.Forsupplychaincontinuity,weofferanidenticalRPMI1640productmadeinourGrandIslandfacility(61870-036).ThisfacilityisalsoregisteredwiththeFDAasamedicaldevicemanufacturerandcertifiedtotheISO13485standards.