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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Gibco/Advanced DMEM/F-12/10 x 500 mL/12634028
产品编号:12634028
市  场 价:¥8240.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$412.00
品      牌: Gibco
公      司:GIBCO
公司分类:
Gibco/Advanced DMEM/F-12/10 x 500 mL/12634028
商品介绍

Description

AdvancedDMEM/F-12(Dulbecco"sModifiedEagleMedium/Ham"sF-12)isawidelyusedbasalmediumthatallowsthecultureofmammaliancellswithreducedFetalBovineSerum(FBS)supplementation.ComparedtoclassicDMEM/F-12,serumsupplementationcanbereducedby50–90%withnochangeingrowthrateormorphology.CellssuccessfullyculturedinAdvancedDMEM/F-12,withnoadaptation,includeMRC-5,SP2,Vero,WI-38andJurkat.

ThisAdvancedDMEM/F-12ismanufacturedasfollows:
WithWithout
•Glucose•L-glutamine
•NonEssentialAminoAcids•HEPES
•Sodiumpyruvate
•PhenolRed

Thecompleteformulationisavailable.

UsingAdvancedDMEM/F-12
AdvancedDMEM/F-12isuniquefromothermediaduetoadditionofthefollowingingredientstoallowforserumreduction:ethanolamine,glutathione,ascorbicacid,insulin,transferrin,AlbuMAX®IIlipid-richbovineserumalbuminforcellculture,andthetraceelementssodiumselenite,ammoniummetavanadate,cupricsulfate,andmanganouschloride.AdvancedDMEM/F-12requiressupplementationwith1–5%FetalBovineSerumand4mML-glutamineorGlutaMAX™supplement.Manycelllinesdonotrequireadaptationtothismedia.TheFBSconcentrationmustbeoptimizedforeachcelllinetoobtainmaximumserumreduction.AdvancedAdvancedDMEM/F-12usesasodiumbicarbonatebuffersystem(3.7g/L)andthereforerequiresa5–10%CO2environmenttomaintainphysiologicalpH.

cGMPmanufacturingandqualitysystem
Forsupplychaincontinuity,wemanufactureAdvancedDMEM/F-12attwoseparatefacilitieslocatedinGrandIsland,NYandScotland,UK.BothsitesarecompliantwithcGMPmanufacturingrequirements,arecertifiedtoISO13485,andareregisteredwiththeFDAasmedicaldevicemanufacturers.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

Specifications

Glutamine:NoGlutamine
PhenolRedIndicator:PhenolRed
Form:Liquid
HEPESBuffer:NoHEPES
SodiumPyruvateAdditive:SodiumPyruvate
SerumSupplementation:Advanced(ReducedSerum)
Glucose:HighGlucose
ProductLine:Gibco®
ProductSize:500mL
Volume(Metric):500ml
GreenFeatures:Sustainablepackaging
ShippingCondition:RoomTemperature

Contents&storage

Storageconditions:2-8°C.Protectfromlight
Shippingconditions:Ambient
Shelflife:12monthsfromdateofmanufacture

Mediaformulations

12634-AdvancedD-MEM/F-12
品牌介绍

GIBCO®是Thermofisher公司的一个重要品牌,1962年诞生于美国纽约的大岛(Grand Island)。致力于细胞培养产品的研发和生产,是世界上最早也是最大的细胞培养产品的供应商,是细胞培养领域全球标准的制定者。

Gibco®血清凭借其一致的品质、超级可靠性和广受赞誉的支持服务,赢得全球研究人员的一致信任。Gibco®血清可满足您的研究需求及预算控制需要,为您最大限度挖掘基础细胞培养、专业研究和特殊试验的价值。我们致力于为您的特定细胞培养需求和实验室预算控制需要提供最适宜的胎牛血清(FBS)产品。

Gibco产品拥有 50 年历史,将科学与经验进行融合从而重新定义了细胞培养的可能性。Gibco提供了一系列创新型高性能产品和基于专业知识的服务,包括快速灵活的细胞系开发、培养基和补料、一流的技术、高质量的定制培养基开发和培养基优化服务。 

Gibco作为细胞培养领域值得信赖的品牌,持续在行业内树立高基准。


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