Description

AdvancedDMEM/F-12(Dulbecco"sModifiedEagleMedium/Ham"sF-12)isawidelyusedbasalmediumthatallowsthecultureofmammaliancellswithreducedFetalBovineSerum(FBS)supplementation.ComparedtoclassicDMEM/F-12,serumsupplementationcanbereducedby50–90%withnochangeingrowthrateormorphology.CellssuccessfullyculturedinAdvancedDMEM/F-12,withnoadaptation,includeMRC-5,SP2,Vero,WI-38andJurkat.


Thecompleteformulationisavailable.

UsingAdvancedDMEM/F-12
AdvancedDMEM/F-12isuniquefromothermediaduetoadditionofthefollowingingredientstoallowforserumreduction:ethanolamine,glutathione,ascorbicacid,insulin,transferrin,AlbuMAX®IIlipid-richbovineserumalbuminforcellculture,andthetraceelementssodiumselenite,ammoniummetavanadate,cupricsulfate,andmanganouschloride.AdvancedDMEM/F-12requiressupplementationwith1–5%FetalBovineSerumand4mML-glutamineorGlutaMAX™supplement.Manycelllinesdonotrequireadaptationtothismedia.TheFBSconcentrationmustbeoptimizedforeachcelllinetoobtainmaximumserumreduction.AdvancedAdvancedDMEM/F-12usesasodiumbicarbonatebuffersystem(3.7g/L)andthereforerequiresa5–10%CO₂environmenttomaintainphysiologicalpH.

cGMPmanufacturingandqualitysystem
Forsupplychaincontinuity,wemanufactureAdvancedDMEM/F-12attwoseparatefacilitieslocatedinGrandIsland,NYandScotland,UK.BothsitesarecompliantwithcGMPmanufacturingrequirements,arecertifiedtoISO13485,andareregisteredwiththeFDAasmedicaldevicemanufacturers.