Description

MinimumEssentialMedium(MEM)isoneofthemostcommonlyusedofallcellculturemedia.MEMcanbeusedwithavarietyofsUSPensionandadherentmammaliancells,includingHeLa,BHK-21,293,HEP-2,HT-1080,MCF-7,fibroblasts,andprimaryratastrocytes.WeofferavarietyofGibco®MEMmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
Thecompleteformulationisavailable.

Gibco®MEM,developedbyHarryEagle,wasbasedonhisearlierformulationofBasalMediumEagle(BME).ManyothermodificationsofMEMfollowed,includingGlasgow’sMEM,MEMα,DMEM,andTemin’sModification.MEMisavailablewithEarle’ssaltsforuseinaCO₂incubator,orwithHanks"saltsforusewithoutCO₂.Gibco®MEMwithGlutaMAX™supplementminimizestoxicammoniabuild-upandimprovescellviABIlityandgrowthinaneasy-to-useformat.ThisproductismadewithEarle’ssalts.

ProductIntendedUse
Forinvitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.

cGMPManufacturingandQualitySystem
Gibco®MEMismanufacturedatacGMPcompliantfacility,locatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferanidenticalGibco®MEMproductmadeinourScotlandfacility(41090-028).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.

MEMcontainsnoproteins,lipids,orgrowthfactors.Therefore,MEMrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).MEMusesasodiumbicarbonatebuffersystem(2.2g/L)andthereforerequiresa5–10%CO₂environmenttomaintainphysiologicalpH.