Description
MinimumEssentialMedium(MEM)isoneofthemostcommonlyusedofallcellculturemedia.MEMcanbeusedwithavarietyofs
USPensionandadherentmammaliancells,includingHeLa,BHK-21,293,HEP-2,HT-1080,MCF-7,fibroblasts,andprimaryratastrocytes.Weofferavarietyof
Gibco®MEMmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisMEMismodifiedasfollows:
With | Without |
•NEAA | •HEPES |
•L-glutamine | •SodiumBicarbonate |
Thecompleteformulationisavailable.
Gibco®MEM,developedbyHarryEagle,wasbasedonhisearlierformulationofBasalMediumEagle(BME).ManyothermodificationsofMEMfollowed,includingGlasgow’sMEM,MEMα,DMEM,andTemin’sModification.MEMisavailablewithEarle’ssaltsforuseinaCO
2incubator,orwithHanks"saltsforusewithoutCO
2.ThisproductismadewithEarle’ssalts.
ProductIntendedUseFor
invitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.
cGMPManufacturingandQualitySystemForsupplychaincontinuity,wemanufactureGibco®MEMattwoseparatefacilitieslocatedinGrandIsland,NYandScotland,UK.BothsitesarecompliantwithcGMPmanufacturingrequirements,arecertifiedtoISO13485,andareregisteredwiththeFDAasmedicaldevicemanufacturers.
MEMcontainsnoproteins,lipids,orgrowthfactors.Therefore,MEMrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).MEMusesasodiumbicarbonatebuffersystem(2.2g/L)andthereforerequiresa5–10%CO
2environmenttomaintainphysiologicalpH.PowderformsofGibco®cellculturemediumrequiresodiumbicarbonatesupplementation,pHadjustment,andfiltrationatthetimeofpreparation(seeprotocolfordetails).
ForInVitroDiagnosticUse.