Description

MinimumEssentialMedium(MEM)isoneofthemostcommonlyusedofallcellculturemedia.MEMcanbeusedwithavarietyofsUSPensionandadherentmammaliancells,includingHeLa,BHK-21,293,HEP-2,HT-1080,MCF-7,fibroblasts,andprimaryratastrocytes.WeofferavarietyofGibco®MEMmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
Thecompleteformulationisavailable.

Gibco®MEM,developedbyHarryEagle,wasbasedonhisearlierformulationofBasalMediumEagle(BME).ManyothermodificationsofMEMfollowed,includingGlasgow’sMEM,MEMα,DMEM,andTemin’sModification.Toallowautoclavingthismedium,cholinechlorideissubstitutedwithcholinebitartrate,phenolredisreduced,andsuccinicacidisaddedtopreventprecipitationatlowpH.ThisMEMformulationcontainsEarle’ssaltsforuseinaCO₂incubator.ThisproductismadewithEarle’ssalts.

ProductIntendedUse
Forinvitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.

Dual-SitecGMPManufacturing
Forsupplychaincontinuity,wemanufactureGibco®MEMattwoseparatefacilitieslocatedinGrandIsland,NYandScotland,UK.BothsitesarecompliantwithcGMPmanufacturingrequirements,arecertifiedtoISO13485,andareregisteredwiththeFDAasmedicaldevicemanufacturers.

MEMcontainsnoproteins,lipids,orgrowthfactors.Therefore,MEMrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).MEMusesasodiumbicarbonatebuffersystem(2.2g/L)andthereforerequiresa5-10%CO₂environmenttomaintainphysiologicalpH.

ThisautoclavableformofGibco®cellculturemediumrequirespHadjustmentto4.1-4.2beforeautoclaving,followingbysodiumbicarbonateandL-glutaminesupplementationandfinalpHadjustmentto7.2–7.4(seeprotocolfordetails).