商品介绍
Description
Lipofectamine®3000TransfectionReagentleveragesourmostadvancedlipidnanoparticletechnologytoprovidesuperiortransfectionperformancewithimprovedapplicationoutcomesandreproducIBLeresults.ThisreagentdeliverssuperiortransfectionefficiencyandimprovedcellviABIlityforthewidestrangeofhard-to-transfectandcommoncells(e.g.,HEK293,HeLa).SuccessfullytransfectthewidestvarietyofBIOLOGicallyrelevantcelltypeswithareagentthatoffers:
•Superiorperformance—ourhighestefficiencyreagentfordifficult-to-transfectcells
•Improvedcellviability—gentleonyourcells,withlowtoxicity
•Versatile—onereagentforDNA,RNA,andco-transfection
EfficientlyTransfectDifficult-to-TransfectCells
Lipofectamine®3000reagentisdesignedtoefficientlytransfectdifficult-to-transfectcells,yieldingsuperiortransfectionperformanceacrossthebroadestarrayofcelltypes.Successfullytransfectedcelltypesinclude:
| Fibroblast(3T3,COS-7) | Myoblast(C2C12,L6CRL-1458) |
| Hepatocyte(HepG2,HuH7) | Erythroleukemiccell(K562) |
| Breastcarcinoma(MCF7,Hs578T) | Prostatecarcinoma(LNCap) |
| Lungcarcinoma(A549,NCI-H460) | Osteosarcoma(U-2OS,Saos-2) |
| Coloncarcinoma(Caco2,SW480) | Pancreaticcarcinoma(PANC-1) |
| Skinmelanoma(SK-MEL-28) |
Viewperformancedatafor60+celllinespluscomparisontoLipofectamine2000>
TransfectCommonCellsEfficientlyandCost-Effectively
Forcommoncelltypes,Lipofectamine®3000reagentismorecost-effectivethanotherreagentsduetoitshighconcentrationandlowusageamount.The1.5mLsizeprovidesupto1500transfectionreactions(in24-wellplates).
AchieveSuperiorTransfectionEfficiencyWhileMaintainingLowToxicity
Lipofectamine®3000reagentmaintainsahightransfectionefficiencywithinarobustdynamicrangeoflipiddosesforquickandeasyoptimization.Alowtoxicitylipiddose(0.75µLfor24-wellplates)issuggestedforapplicationsrequiringminimaldisruptionofthecells.
EnableHighTransfectionEfficiencyinNovelGenomeEditingApplications
Lipofectamine®3000reagentissuitablefornovelgenomeeditingapplications.ItincreasesthelikelihoodofsuccessfulcleavageandrecombinationwithTALENsorCRISPRsthroughhighlyefficienttransfection,ultimatelymaximizingtheefficiencyofgeneticmodificationsandsimplifyingthedownstreamprocesses.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Figures
Part1:BetterperformanceinanarrayofcelllinescomparedtoLipofectamine®2000reagent
Lipofectamine®3000transfectionproceduredetails
Lipofectamine®3000transfectionworkflow
EnhancedtransfectionefficiencyusingLipofectamine®3000
Part2:BetterperformanceinanarrayofcelllinescomparedtoLipofectamine®2000reagent
CleavageefficiencyforGeneArt®TALENsandCRISPRs
Specifications
| CellType: | EstablishedCellLines, Hard-to-TransfectCells, PrimaryCells, StemCells |
|---|---|
| SampleType(Specific): | PlasmidDNA, RNAiPlasmids(shRNA,miR), SyntheticsiRNA |
| Classification: | AnimalOrigin-Free |
| TransfectionTechnique: | Lipid-BasedTransfection |
| SerumCompatible: | Yes |
| ProductLine: | Lipofectamine® |
| ProductSize: | 0.1mL |
Contents&storage
ContainsonevialofLipofectamine®3000ReagentandonevialofP3000TMEnhancerReagent.Storeat2–8°C.Donotfreeze.
品牌介绍
GIBCO®是Thermofisher公司的一个重要品牌，1962年诞生于美国纽约的大岛(Grand Island)。致力于细胞培养产品的研发和生产，是世界上最早也是最大的细胞培养产品的供应商，是细胞培养领域全球标准的制定者。
Gibco®血清凭借其一致的品质、超级可靠性和广受赞誉的支持服务，赢得全球研究人员的一致信任。Gibco®血清可满足您的研究需求及预算控制需要，为您最大限度挖掘基础细胞培养、专业研究和特殊试验的价值。我们致力于为您的特定细胞培养需求和实验室预算控制需要提供最适宜的胎牛血清(FBS)产品。
Gibco产品拥有 50 年历史，将科学与经验进行融合从而重新定义了细胞培养的可能性。Gibco提供了一系列创新型高性能产品和基于专业知识的服务，包括快速灵活的细胞系开发、培养基和补料、一流的技术、高质量的定制培养基开发和培养基优化服务。
Gibco作为细胞培养领域值得信赖的品牌，持续在行业内树立高基准。
公司介绍
产品分类
Hepatocytes
2-Mercaptoethanol
F-12 Nutrient Mixture
Geltrex Basement Membranes
Mammalian Expression Kits
TrypLE
Extracellular Matrix Proteins
Water
Serum From Other Blood
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Earle\'s Balanced Salt Solution
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Hanks\' Balanced Salt Solution
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293 Media
Cryopreservation Media
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Iscove\'s (IMDM)
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Opti-MEM I
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Serum Replacements
Insulin, Transferrin, & Iron Supplements
Other Supplements
Stem Cell Differentiation Kits
Phosphate-Buffered Salines
Electroporation Instruments
CHO & DG44 Media
Mammalian Cell Lines
Leibovitz\'s L-15
Schneider\'s Drosophila Media
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CIF100%正规报关，提供发票
及时交付
限时必达 不达必赔
总部地址：
江苏省苏州市工业园区星湖街218号 苏州生物医药产业园一期（生物纳米园）A4-216(邮编：215125)
工作时间：周一至周五9:00~18:00
技术支持：
短信：18915418616
服务热线：
4000-520-616（全国）
☏ 0512-67156496（苏州）
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