Description
Opti-MEM®IReduced-SerumMediumisanimprovedMinimalEssentialMedium(MEM)thatallowsforareductionofFetalBovineSerumsupplementationbyatleast50%withnochangeingrowthrateormorphology.Opti-MEM®Imediumisalsorecommendedforusewithcationiclipidtransfectionreagents,suchasLipofectamine™reagent.Opti-MEM®Imediumcanbeusedwithavarietyofs
USPensionandadherentmammaliancells,includingSp2,AE-1,CHO,BHK-21,HEK,andprimaryfibroblasts.WeofferavarietyofOpti-MEM®Imodificationsforarangeofcellcultureapplications.
ThisOpti-MEM®Iismodifiedasfollows: Thecompleteformulationisconfidential.Formoreinformation,pleasecontactTechnicalServices.
UsingOpti-MEM®IMediumOpti-MEM®IReduced-SerumMediumisauniquemediumthatcontainsinsulin,transferrin,hypoxanthine,thymidine,andtraceelements.Theseadditionalcomponentsallowforareductioninserumsupplementationbyatleast50%.Opti-MEM®Imediumusesasodiumbicarbonatebuffersystem(2.4g/L),andthereforerequiresa5–10%CO
2environmenttomaintainphysiologicalpH.
cGMPmanufacturingandqualitysystemOpti-MEM®IismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferanidenticalOpti-MEM®IproductmadeinourScotlandfacility(51985-042).ThisfacilityisalsoregisteredwiththeFDAasamedicaldevicemanufacturerandcertifiedtotheISO13485standard.
ForInVitroDiagnosticUse.