商品介绍
Description
FreeStyle™CHOExpressionMediumisananimalorigin–free,chemicallydefined,protein-freemediumspecificallydevelopedtosupportthegrowthandtransienttransfectionofCHO-ScellsinsUSPensionwithoutadaptation.Thismediumrequiressupplementationwith8mMglutaminebeforeuse.Gibco®FreeStyle™CHOExpressionMediumfeatures:
•Formulationwithouthypoxanthineandthymidineforusewithorwithoutdhfrsystems
•ABIlitytosupporthigh-densitysuspensiongrowthandtransienttransfectionofCHO-Scells
•Animalorigin–free,protein-freeandchemicallydefinedformulation
•EasyuseaspartoftheFreeStyle™MAXCHOExpressionSystemforproteinproduction
•Scalabilityinspinnerflasksandbioreactors
Formulationwithouthypoxanthineandthymidineforusewithorwithoutdhfrsystems
Gibco®FreeStyle™CHOExpressionMediumismadewithouthypoxanthineandthymidineforuseindihydrofolatereductase(dhfr)amplificationsystems.Non-dhfrsystemsrequiretheadditionofHTSupplementbeforeuse.
Abilitytosupporthigh-densitysuspensiongrowthandtransienttransfectionofCHO-Scells
Gibco®FreeStyle™CHOcellsinsuspensioncanbecultureddirectlyintoGibco®FreeStyle™CHOExpressionMediumwithoutadaptation.Cellsadaptedtoothercommerciallyavailableserum-freemediacanbesubcultureddirectlyintoGibco®FreeStyle™CHOExpressionMedium,usuallywithoutanyfurtheradaptation.Cellsusuallyrequireadaptationfromserum-containingformulations.CellsculturedinGibco®FreeStyle™CHOExpressionMediumcanbetransfectedusinglipid-basedtransfectionreagents,suchasFreestyle™MAXreagent.
Animalorigin–free,protein-freeandchemicallydefinedformulation
Gibco®FreeStyle™CHOExpressionMediumisanimalorigin–free,protein-free,andchemicallydefined,allowingforeasierpurificationofyourproteinofinterest.Gibco®chemicallydefinedmediacontainnoproteins,hydrolysates,orcomponentsofunknowncomposition.
EasyuseaspartoftheFreeStyle™MAXCHOExpressionSystemforproteinproduction
TheFreeStyle™MAXCHOExpressionSystemisabreakthroughtechnologyforrapid(seefigure)andhigh-yieldmammalianproteinproductionin30mLand1Lbatchsizes(seefigure).TheFreeStyle™MAXCHOExpressionSystemcombinesGIBCO®FreeStyle™media,FreeStyle™MAXreagent,andeitherFreeStyle™CHO-ScellsorFreeStyle™293-Fcellsinonekit.
Scalabilityinspinnerflasksandbioreactors
ProteinproductionusingGibco®FreeStyle™CHOExpressionMediumcanbescaledupinspinnerflasksorbioreactors.Theappropriatespinnerorimpellerspeedandseedingdensityshouldbeoptimizedforeachsystem.Dependingontheimpellerdesignandspeed,itmaybenecessarytosupplementGibco®FreeStyle™CHOExpressionMediumwithadditionalPluronic®F-68toavoidsheerstressintheculture.
cGMPmanufacturingandqualitysystem
Gibco®FreeStyle™CHOExpressionMediumismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Figures
Proteinproductioncomparison—suspensionCHOcellsandstablecelllinesystems.
ProteinproductionaftertransienttransfectionofFreeStyleâ„¢CHOCells.
Specifications
| CellLine: | CHO, FreeStyle™CHO-SCells |
|---|---|
| Classification: | ChemicallyDefined, Protein-Free, Serum-Free |
| CultureType: | SuspensionCellCulture |
| EndotoxinLevel: | ≤0.25EU/ml |
| Form: | Liquid |
| Format: | Bottle(s) |
| Glutamine: | NoGlutamine |
| PhenolRedIndicator: | NoPhenolRed |
| ProductLine: | Freestyle™, Gibco® |
| ProductSize: | 1,000mL |
| SerumLevel: | Serum-Free |
| GreenFeatures: | Sustainablepackaging |
| ShippingCondition: | RoomTemperature |
Contents&storage
Storageconditions:2°Cto8°C.Protectfromlight
Shippingconditions:Ambient
Shelflife:12monthsfromdateofmanufacture
Shippingconditions:Ambient
Shelflife:12monthsfromdateofmanufacture
品牌介绍
GIBCO®是Thermofisher公司的一个重要品牌，1962年诞生于美国纽约的大岛(Grand Island)。致力于细胞培养产品的研发和生产，是世界上最早也是最大的细胞培养产品的供应商，是细胞培养领域全球标准的制定者。
Gibco®血清凭借其一致的品质、超级可靠性和广受赞誉的支持服务，赢得全球研究人员的一致信任。Gibco®血清可满足您的研究需求及预算控制需要，为您最大限度挖掘基础细胞培养、专业研究和特殊试验的价值。我们致力于为您的特定细胞培养需求和实验室预算控制需要提供最适宜的胎牛血清(FBS)产品。
Gibco产品拥有 50 年历史，将科学与经验进行融合从而重新定义了细胞培养的可能性。Gibco提供了一系列创新型高性能产品和基于专业知识的服务，包括快速灵活的细胞系开发、培养基和补料、一流的技术、高质量的定制培养基开发和培养基优化服务。
Gibco作为细胞培养领域值得信赖的品牌，持续在行业内树立高基准。
公司介绍
产品分类
Hepatocytes
2-Mercaptoethanol
F-12 Nutrient Mixture
Geltrex Basement Membranes
Mammalian Expression Kits
TrypLE
Extracellular Matrix Proteins
Water
Serum From Other Blood
General Purpose Bottles
Earle\'s Balanced Salt Solution
DMEM Media
Extracellular Matrix Coated Plates
Hanks\' Balanced Salt Solution
Other Cell Dissociation Reagents
293 Media
Cryopreservation Media
DMEM⁄F-12 Media
Minimum Essential Media (MEM)
Transfection Reagents
Trypsin
Iscove\'s (IMDM)
AlgiMatrix Bioscaffolds
Dulbecco\'s Phosphate-Buffered Salines
Other Serum-Free & Specialty Media
Other General Chemicals
Opti-MEM I
Growth Factors, Chemokines & Cytokines
Electroporation Instrument Accessories
Antibiotics & Antimycotics
RPMI Medium 1640
HEPES Buffer
Neuronal Cell Media
Fetal Bovine Serum
Serum Replacements
Insulin, Transferrin, & Iron Supplements
Other Supplements
Stem Cell Differentiation Kits
Phosphate-Buffered Salines
Electroporation Instruments
CHO & DG44 Media
Mammalian Cell Lines
Leibovitz\'s L-15
Schneider\'s Drosophila Media
销售排行
厂家直采
全球直采 正品优价
正品保障
厂家直发 有线跟踪
正规清关
CIF100%正规报关，提供发票
及时交付
限时必达 不达必赔
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