Description

ExpiCHO™ExpressionMediumisaGMP-manufactured,chemically-defined,serum-free,protein-free,animalorigin–freemediumspecificallydesignedforhigh-densitygrowthandtransfectionofsUSPension-adaptedChinesehamsterovary(CHO)cells.ItisacorecomponentoftheExpiCHO™ExpressionSystemKit(Cat.No.A29133)forscalabletransientproteinproduction.

ExpiCHOExpressionMedium:
•SupportsgrowthofsuspensionCHOculturestodensitiesofover2x10⁷cellspermL,with>95%cellviABIlity
•Usesatransfection-compatible,serum-freesuspensionculturemediumthatenableshightransfectionefficienciesof>90%withExpiFectamine™CHOtransfectionreagent
•SupportstransienttransfectionofsuspensionCHOcellsatadensityof6x10⁶cellspermL
•Facilitatessustained,high-levelexpressionofhigh-densitytransientlytransfectedcultures,achievingyieldsofupto3gperLofrecombinantprotein
•SupportsgrowthandtransfectionofCHOcellsincultureformatsfrom<1 ml="" in="" multi-well="" plates="" to="">10Linbenchtopbioreactors
•Doesnotcontainphenolred

ExpiCHOExpressionMediumisformulatedwithGlutaMAX™-Ireagent.Itisreadytouse,withnosupplementationrequired.Thechemically-definedformulationresultsinhighreproducibilityandlot-to-lotreliability.Forhighestleveltransientproteinexpression,themediumisusedincombinationwithamatchedculturefeed,ExpiCHO™Feed,whichissuppliedaspartoftheExpiFectamine™CHOTransfectionKit(Cat.No.A29129).ExpiCHOExpressionMediumisnotrecommendedforadherentcellcultures.CellsaretypicallygrowninErlenmeyer-styleflasksonashakerplatforminahumidified,CO₂cellcultureincubator.However,culturesarescalablefromvolumesof<1 ml="" in="" multi-well="" plates="" to="">10Linbioreactors.