Description
HepExtend™Supplement(50X)hasbeenoptimizedforusewithcryopreservedprimaryhepatocytestoimprovecellvi
ABIlity,function,andnumberofdaysinculture.Thisenablesresearcherstoperformmetabolicandtoxicityexperimentsnotachievableusingstandardcultureconditions.SimplyaddHepExtend™SupplementtostandardWilliam’sEHepatocyteMaintenanceMediaandusewithyourcurrenthepatocyteculturingmethodsandinstrumentation.Thesupplementdoesnotcontainanysmallmoleculesorfetalbovineserum,componentsthatareknowntointerferewithprimaryhepatocytefunction.
•Enabling—designedtoprovidemaximumcellviabilityandcellhealthwhenculturingprimaryplateablehepatocytes
•Extendedcellsurvival—daysinculturecanbeextendedto>10dayswhilemaintainingnormalcellmorphologyandbilecanaliculi
•Consistent—manufacturedinacGMP-compliantfacilityusingthehighestqualitymaterials
MaintainP450activityHepExtend™Supplementcanbeusedwithyourstandardhepatocyteculturemediatohelpfacilitatemetabolicallycompetenthepatocytesforatleast10daysforuseindownstreamADME/Toxapplicationsnotachievablewithstandard5-dayculturesystems.
EnhancedgeneexpressionHepExtend™supplementationincreasesthebasalgeneexpressionofmanykeygenesinvolvedindrugmetabolismandtransport.
RetaininducibilityNuclearreceptoractivationandgeneinducibilityisretainedinculturestreatedwithHepExtend™supplementedmedia.
ForResearchUseOnly.Notforuseindiagnosticprocedures.