Description
Geltrex™LDEV-FreeReducedGrowthFactorBasementMembraneMatrixisasolubleformofbasementmembraneextractedfrommurineEngelbreth-Holm-Swarm(EHS)tumorsandisfreeofviruses,includinglactosedehydrogenaseelevatingvirus(LDEV),sometimesfoundinthistypeofpreparation.ThemajorcomponentsofGeltrex™matrixincludelaminin,collagenIV,entactin,andheparinsulfateproteoglycans.Itisidealforthepromotionandmaintenanceofdifferentiatedphenotypesinavarietyofcellculturesincludingprimaryepithelialcells,endothelialcells,smoothmusclecells,andhumaninducedpluripotentstemcells(iPSC).Geltrex™matrixoffers,onaverage,a2-foldreductioninseveralgrowthfactors.Asaresult,analysisofspecificgrowthfactoreffectsinapplicationsrequiringbasementmembraneextractcanbecarriedoutinacontrolledandreproduc
IBLeformat.
KeyFeaturesofGeltrex™LDEV-FreeReducedGrowthFactorBasementMembraneMatrix:•Freeoflactosedehydrogenaseelevatingvirus(LDEV)makingitidealforalltypesofcellcultureandmouse
invivoapplications
•Consistentproteinconcentrationlot-to-lothelpsreducetheneedtoscreenlots
•Protocolsforvariousapplications,includingtubeformationassays,areavailable
GainReliABIlityandConsistencyinYourhESCStudiesRecentadvancesinprocessingEHStumorcellshaveresultedinvirusfreebasementmembraneextracts.EverylotistestedbyRT-PCRmethodssoyoucannowhaveconfidencethateverylotofGeltrex™isvirusfree.Additionally,ourconsistentmanufacturingprocesshelpsensureaproteinconcentrationwithverylittlevariationfromlot-to-lot,averaging15mg/mlsoyoucanhaveconfidenceineverypurchase.
InvestigateAngiogenesiswithValidatedGibco®ProtocolsTherearemanyapplicationsforGeltrex™LDEV-Freereducedgrowthfactorbasementmembranematrix,eachrequiringdifferentthicknessesandconcentrations.Ingeneral,aproteinconcentrationof>5mg/mlisusedfordifferentiationstudiesofprimarycells.Geltrex™LDEV-FreereducedgrowthfactorbasementmembranematrixhasbeenusedtodeveloptubeformationassayprotocolsusingGibco®primarycellsandspecialtymedia.Seethisandotherangiogenesisassayprotocols
ForResearchUseOnly.Notforuseindiagnosticprocedures.